APR September/October 2020 - 162

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MICROBIOLOGY

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4.

Nakamura S, Morita T, Iwanaga S. Biochemical characterization of protein components
associated with hemolymph coagulation system of limulus (Tachypleus tridentalus).
Thrombosis and Haemostasis. 1983;50: 405.

24.

Ding JL, Navas MAA III, Ho B. Molecular cloning and sequence analysis of Factor C cDNA
from the Singapore horseshoe crab, Carcinoscorpius rotundicauda. Molecular Marine Biol
and Biotechnol. 1995;4(1):90-103.

5.

Iwanaga S, Morita T, Miyata T, Nakamura T. Hemolymph coagulation system in Limulus.
In: L. Leive et al. ed. Microbiology, American Soc. Microbiology. Washington. 1985:pp.2932.

25.

Pui AWM, Ho B, Ding JL. Yeast recombinant Factor C from horseshoe crab binds endotoxin
and causes bacteriostasis. J Endotoxin Res. 1997;4:391-400.

26.

6.

Navas MAA III, Ding JL, Ho B. Inactivation of Factor C by dimethyl sulfoxide inhibits
coagulation of the Carcinoscorpius amoebocyte lysate. Biochem Intl. 1990;21(5):805-813.

Ding JL, Chai C, Pui AWM, Ho B. Expression of full length and deletion homologues of
Carcinoscorpius rotundicauda Factor C in Sacharromyces cerevisiae: immunoreactivity and
endotoxin binding. J. Endotoxin Research 1997;4(1):33-43.

7.

Muta T, Miyata T, Misumi Y, Tokunaga F, Nakamura T, Toh Y, Ikehara Y, Iwanaga S. Limulus
factor C: an endotoxin-sensitive serine protease zymogen with a mosaic structure of
complement-like, epidermal growth factor-like, and lectin-like domains. J Biol Chem
1991;266(10):6554-6561.

27.

Roopashree SD, Ho B, Ding JL. Expression of Carcinoscorpius rotundicauda Factor C in Pichia
pastoris. Mol Mar Biol Biotechnol. 1996;5(4):334-343.

28.

Roopashree SD, Ho B, Ding JL. Recombinant COS-1 cells express Carcinoscorpius
rotundicauda Factor C. Biotechnol Lett. 1997;19:357-361.

29.

Wang J, Ho B, Ding JL. Functional expression of full length Limulus Factor C in stably
transformed Sf9 cells. Biotechnol Lett. 2001;23:71-76.

30.

Ding JL, Ho B. A new era in pyrogen testing. Trends Biotechnol. 2001) 19(8):277-281

31.

Wang LH, Ho B, Ding JL. Transcriptional regulation of Limulus Factor C. J. Biol Chem.
2003;278(49):49428-49437.

32.

Ding JL, Tan KC, Thangamani S, Kusuma N, Seow WK, Bui THH, Wang J, Ho B. Spatial and
temporal coordination of expression of immune response genes during Pseudomonas
aeruginosa infection of horseshoe crab, Carcinoscorpius rotundicauda. Genes and
Immunity 2005; doi:10.1038/sj.gene.6364240.

8.

Ho B, Kim JC, Ding JL. Electrophoretic analysis of endotoxin-activated gelation reaction of
Carcinoscorpius rotundicauda amoebocyte lysate. Biochem Mol Biol Int. 1993;29(4):687-
694.

9.

Ding JL, Navas MAA III, Ho B. Two forms of factor C from the amoebocytes of Carcinoscorpius
rotundicauda: purifi cation and characterisation. Biochim Biophys Acta 1993;1202(1):149-
156.

10.

Ding JL, Sababathy TK, Ho B. Morphological changes of Carcinoscorpius rotundicauda
amoebocyte and Escherichia coli during their interaction. Cytobios 1993;75(300):21-32.

11.

Iwanaga S, Muta T, Shigenaga T, Miura Y, Seki N, Saito T, Kawabata S. Role of hemocytederived granular components in invertebrate defense. Ann NY Acad Sci. 1994;712:102-
116.

33.

12.

Ding JL, Navas MA 3rd, Ho B. Molecular cloning and sequence analysis of factor C cDNA from
the Singapore horseshoe crab, Carcinoscorpius rotundicauda. Mol Mar Biol Biotechnol.
1995;4(1):90-103.

Ding JL, Ho B. Endotoxin detection - from Limulus amebocyte lysate to recombinant
factor C. In: X. Wang, PJ. Quinn eds. Endotoxins: Structure, Function and Recognition,
Subcellular Biochem 2010;53:187-208.

34.

13.

Muta T, Iwanaga S. The role of hemolymph coagulation in innate immunity. Curr Opin
Immunol 1996;8(1):41-47

Yeo DSA, Ding JL, Ho B. Neuroblastoma cell culture assay shows that Carcinoscopius
rotundicauda haemolymph neutralizes tetrodotoxin. Toxicon 1996;34(9):1054-1057.

35.

14.

Wang J, Tan NS, Ho B, Ding JL. Modular arrangement and secretion of a multidomain serine
protease: evidence for involvement of proline-rich region and N-glycans in the secretion
pathway. J Biol Chem. 2002;277(39):36363-36372.

Ng PM, Le Saux A, Lee CM, Tan NS, Lu JH, Thiel S, Ho B, Ding JL. C-reactive protein
collaborates with plasma lectins to boost immune response against bacteria. EMBO J.
2007;26(14):3431-3440.

36.

15.

Schmidtke M, Eyler S, Michels S, Wright C, Ebbin S. 2019 Review of the Atlantic States
Marine Fisheries Commission Fishery Management Plan for Horseshoe Crab. Atlantic
States Marine Fisheries Commission. Oct. 2019. http://www.asmfc.org/uploads/
file/5e0fa319HSC_FMPReview_2019.pdf

Nagai T, Osaki T, Kawabata S. Functional conversion of hemocyanin to phenoloxidase by
horseshoe crab antimicrobial peptides. J Biol Chem. 2001;276(29):27166-27170.

37.

Jiang N, Tan NS, Ho B, Ding JL. Respiratory protein-generated reactive oxygen species as an
antimicrobial strategy. Nature Immunol. 2007;8(10):1114-1122.

38.

Zhu Y, Thangamani S, Ho B, Ding JL. The ancient origin of the complement system. EMBO
J. 2005;24(2):382-394

39.

Zhu Y, Ng PM, Wang L, Ho B, Ding JL. Diversity in lectins enables immune recognition and
differentiation of wide spectrum of pathogens. Int Immunol. 2006;18(12):1671-1680

40.

Li Y, Ng PML, Ho B, Ding JL. A female-specific pentraxin, CrOctin, bridges pattern recognition
receptors to bacterial phosphoethanolamine. Eur J. Immunol. 2007:37:3477-3488.

16.

Cramer, D. ed. In: The Narrow Edge: a tiny bird, an ancient crab and an epic journey. Yale
University Press, New Haven and London (ISBN: 978-0-300-18519-5) 2015.

17.

The Atlantic, Science, Zhang S. The Last Days of the Blue-Blood Harvest. Available at:
https://www.theatlantic.com/science/archive/2018/05/blood-in-the-water/559229/
2018; Accessed July 21, 2020

18.

Revive & Restore, The horseshoe crab project. Available at: https://reviverestore.org/
horseshoe-crab/about/ Accessed July 21, 2020.

41.

19.

Akbar JB, Nelson, B.R, Sheikh, H.I., Cheung, S.G., Wardiatno, Y., Dash B.P., Tsuchiya, K.,
Iwasaki, Y. Pati, S. A review on fisheries and conservation status of Asian horseshoe crabs.
Biodiversity and Conservation. 2018 https://doi.org/10.1007/s10531-018-1633-8

Loh BWC, Wang XW, Bui TTH, Luan XL, Ho B, Ding JL. SARM: a novel TLR adaptor, is
functionally conserved from arthropod to human. Mol Immunol. 2008;45:1732-1742.

42.

20.

Krisfalusi-Gannon J, Ali W, Dellinger K, Robertson L, Brady TE, Goddard MKM, TinkerKulberg R, Keple CL, Dellinger AL. The role of horseshoe crabs in the biomedical industry
and recent trends impacting species sustainability. Front. Mar. Sci. (Global Change and the
Future Ocean). 2018; https://doi.org/10.3389/fmars.2018.00185

Jiang N, Thangamani S, Chor CF, Wang SY, Winarsih I, Du RJ, Sivaraman S, Ho B, Ding JL. A
novel serine protease inhibitor acts as an immunomodulatory switch while maintaining
homeostasis. J. Innate Immunity 2009;1:465-479.

43.

Tan NS, Ho B, Ding JL. High-affinity LPS binding domain(s) in recombinant Factor C of a
horseshoe crab neutralizes LPS-induced lethality. FASEB J. 2000;14:859-870.

44.

21.

Maloney T, Phelan R. Simmons N. Saving the horseshoe crab: a synthetic alternative
horseshoe crab blood for endotoxin detection. PLoS Biology 2018;16(10):e2006607.doi
10.1371/journal.pbio.2006607

Tan NS, Ng PML, Yau HY, Chong PKW, Ho B, Ding JL. Definition of endotoxin binding sites
in horseshoe crab Factor C recombinant sushi pproteins and neutralization of endotoxin by
sushi peptides. FASEB J. 2000;14:1801-1813.

45.

22.

Muta T, Seki N, Takaki Y, Hashimoto R, Oda T, Atsufumi I, Tokunaga F, Iwanaga S. Purified
horseshoe crab factor G: reconstitution and characterization of the (1-3)-beta-D-glucansensitive serine protease cascade. J Biol Chem 1995;270(2):892-897

Williams KL. Cleanroom Technology, "Recombinant Factor C assay to aid demand for LAL
endotoxin testing" 9 March 2020. https://www.cleanroomtechnology.com/news/article_page/
Recombinant_Factor_C_assay_to_aid_demand_for_LAL_endotoxin_testing/163099

46.

23.

Rolansky PF, Novitsky TJ. Sensitivity of Limulus amoebocyte lysate (LAL) to LAL-reactive
glucans. J. Clin Microbiol. 1991;29 (11): 2477-2483.

Bolden J, Smith K. Application of recombinant Factor C reagent for the detection of bacterial
endotoxins in pharmaceutical products. PDA J. Pharm Sci Technol 2017;71(5):405-412 doi:
10.5731/pdajpst.2017.007849

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| September/October 2020

10/2/20 11:46 AM
http://www.asmfc.org/uploads/file/5e0fa319HSC_FMPReview_2019.pdf https://www.theatlantic.com/science/archive/2018/05/blood-in-the-water/559229/2018 https://reviverestore.org/horseshoe-crab/about/ https://www.doi.org/10.1007/s10531-018-1633-8 https://www.doi.org/10.3389/fmars.2018.00185 https://www.cleanroomtechnology.com/news/article_page/Recombinant_Factor_C_assay_to_aid_demand_for_LAL_endotoxin_testing/163099
APR September/October 2020

Table of Contents for the Digital Edition of APR September/October 2020
