APR Nov/Dec 2022 - 70

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analysis of trace elements in single cells. Scientists can now accurately
establish trace element mass per cell and the element distribution
across a cell cohort.
However, this technique relies heavily on the right analytical software
being in place so that detection and transport efficiencies can both
be determined accurately, and a sequential approach can be used to
measure multiple elements for identical periods on a single sample
aspiration. With comprehensive data evaluation tools, both raw and
intermediate data can then be utilized to enable full representation
and analysis of the mass and number concentration data.
By using scICP-MS technology to detect trace elements at the single-cell
level, analysts can gain a greater understanding of the migration and
function of trace elements at a cellular level. Data such as these can be
used in a variety of applications, perhaps for the development of more
advanced therapies for a wide range of clinical presentations, better
diagnostics for toxin ingestion or environmental pollution and even to
improve quality and efficiency measures in bioproduction processes.
References
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cardiometabolic indices of patients with atherosclerosis: A double-blind randomized
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Thompson PA, Ashbeck EL, et al. Selenium Supplementation for Prevention of Colorectal
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Klein, EA. Selenium and vitamin E cancer prevention trial. Ann. N.Y. Acad. Sci.
2004;1031:234-241.
Gilbert-López B, Dernovics M, et al. Detection of over 100 selenium metabolites in
selenized yeast by liquid chromatography electrospray time-of-flight mass spectrometry.
J. Chromatogr. B. 2017;1060:84-90.
Álvarez-Fernández García R, Corte-Rodríguez, M, et al. Addressing the presence of biologic
selenium nanoparticles in yeast cells: analytical strategies based on ICP-TQ-MS. Analyst.
2020;145:1457-1465.
Thermo Fisher Scientic. Application Note 001349. Assessing the level and distribution of
selenium in selenized yeast cells using single cell ICP-MS analysis. Available at: https://
assets.thermofisher.com/TFS-Assets/CMD/Application-Notes/an-001349-icp-msselenium-yeast-cells-an001349-na-en.pdf
About
the Author
National Institutes of Health Office of Dietary Supplements. Selenium Fact Sheet for
Healthcare Professionals page. Available at: https://ods.od.nih.gov/factsheets/SeleniumHealthProfessional/
Accessed October 19, 2022.
| November/December 2022
Simon Nelms is a former ICP-OES and ICP-MS applications
specialist who's been part of the Thermo Fisher Scientific
team for more than 20 years. He is now the marketing
manager for the ICP-MS product range. Simon holds a
BSc in Analytical Chemistry and a PhD in research involving ICP-MS
method development.

APR Nov/Dec 2022

Table of Contents for the Digital Edition of APR Nov/Dec 2022