Figure 7. Sensitivity assessment on the QuantStudio 5 instrument (top): Results demonstrate a clean separation between LOD and NTC. Specificity assessment on the QuantStudio 5 instrument (bottom): Both Sf9 and Baculovirus assays demonstrate high specificity in the presence of genomic DNA from other sources. matrix similar to the final drug formulation. The spikein was done to mimic extraction of 100 pg, 10 pg, and 1 pg DNA from both of these matrices, as well as a negative control. Results show efficient spike recovery using PrepSEQ with both KingFisher Flex and manual extraction. (Figure 9) (6) Inhibition control/IPC performance: With QuantStudio 5, the IPC performs similarly to the standard curve, in matrices containing inhibitors, such as Benzonase and Tween20, showing that sample prep successfully removes inhibitors from a variety of matrices that are common in bioproduction workflows. (Figure 9) Conclusion The above case studies show clearly that the resDNASEQ Quantitative Sf9 and Baculovirus DNA duplex assay kit for residual DNA testing is a robust, comprehensive solution with a rapid workflow and optimized sample prep. It is a highly sensitive assay with a LOQ as low 30