eBook: Analytical Method and QC Testing Strategies for Biologics Production - 31

as 0.3 pg (300 fg) for both Sf9 and Baculovirus, and it
also performs reliably across multiple sites, multiple
instruments and operators.
Learn more about the Applied Biosystems™
resDNASEQ™ Quantiative Sf9 and Baculovirus DNA Kit.
References
1.
Figure 8. Singleplex vs multiplex performance on QuantStudio
5 instrument: The standard curves to overlap indicating the
Sf9 and Baculovirus assays do not interfere with each other.
Challener, C. A. Managing Residual Impurities During Downstream
Processing. BioPharm Int. 2017.
2. Hu, Y. Baculovirus as a highly efficient expression vector in insect
and mammalian cells. Acta Pharmacol Sin, 2005, 26, 405-416.
Figure 9. Spike recovery on the QuantStudio 5 instrument (top): Results show efficient % spike recovery using PrepSEQ with both KingFisher
Flex and manual extraction. Testing inhibition control on the QuantStudio 5 instrument (bottom): IPC Ct values obtained show that samples
were successfully prepared from a variety of matrices containing inhibitors.
31
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eBook: Analytical Method and QC Testing Strategies for Biologics Production

Table of Contents for the Digital Edition of eBook: Analytical Method and QC Testing Strategies for Biologics Production

eBook: Analytical Method and QC Testing Strategies for Biologics Production - 1
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