eBook: Advanced Tools Transforming Neurology Research - 17

the probe revealed a waveform that closely resembles
the ventricular complex and T wave of normal
electrocardiogram waves. Cerebral organoids were
also evaluated using multi-array electrode probes
to capture the electrical impulses generated from
neurons. The same silicon probe was placed onto
the organoid to capture impulse conduction across
the organoid, revealing a consistent waveform
conducted across the organoid. (Figure 5).
Figure 3. Bulk RNA sequencing of cerebral organoids. Various
gene markers were monitored for the presence of various cell
types and subtypes throughout the growth and maturation of
generated cerebral organoids.
Figure 5. Transient voltage potential across cerebral organoid.
Silicon probes were placed across a cerebral organoid to measure
electrophysiological activity. Neural conductive activity
was observed using these channels identifying the electrical impulses
between neural cells throughout the organoid.
Figure 4. Transient voltage potential across cardiac organoid.
Silicon probes were placed across a cardiac organoid to measure
the impulse generation and propagation causing the contraction
activity. Each contraction lasts around 449 ms and can
be continuously observed.
conduction across the organoid by visualizing the
transient voltage change during each pulse (Figure
4). The resulting electrical waveform captured by
Discussion
Organoids are an up-and-coming disease modeling
technique that can bridge the challenges of
current in vivo/in vitro models with human models
to enable more successful therapeutic development.
By modeling the complex cell-cell behavior
and cellular microenvironment, organoids provide
17

eBook: Advanced Tools Transforming Neurology Research

Table of Contents for the Digital Edition of eBook: Advanced Tools Transforming Neurology Research