eBook: Advanced Tools Transforming Neurology Research - 22

Figure 1. Time-lapse measuring of ATP-dependent calcium efflux. The represented time-lapse images of Fluo-8® fluorescent signals by calcium
mobilization after treatment of 20 µM ATP reagent (A-E). The changes of the total cell fluorescent signal were plotted in (F), and the three instance
cells have individually plotted the changes of calcium flux by the ATP ageists. The color-coordinated arrows were represented on the plot
(G) based on the images (A-E).
to wells should be synchronized among the wells
by using multi-channel pipet. The calcium flux assay
with Fluo-8® AM was perceived as the green
fluorescence as the intensity at Ex/Em = 490/525.
The [calcium flux measurement] pipeline was built
in CELENA® X Analyzer and applicable to measure
total fluorescent and to trace specific cells' intensity
out of the multiple sequential images of either
z-stack or time-lapse in CELENA® X imaging system
of live cell fluorescent measurement.
References
1. Hahn, K., R. DeBiasio, and D.L. Taylor, Patterns of
elevated free calcium and calmodulin activation in
living cells. Nature, 1992. 359(6397): p. 736-8.
2. Vofely, G., et al., Characterization of calcium signals in
human induced pluripotent stem cell-derived dentate
gyrus neuronal progenitors andmmature neurons,
stably expressing an advanced calcium indicator
protein. Mol Cell Neurosci, 2018. 88: p. 222-230.
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