Contents 4 6 New Tools and Techniques Advancing Neuroscience Research Technological advances allow neuroscientists to tackle new challenges. Microscopy Advances Accelerate Neuroscience Research New tech approaches brain imaging in ways that weren't previously possible. 10 Pre-formed Fibrils: Another Perspective in Modeling of Neurodegenerative Diseases With many limitations for current disease models, PFFs may be an efficient, cost-effective strategy. 16 Sonication of Pre-formed Fibrils for Neurodegenerative Disease Research: What, Why, How, and Best Practices Sonication is an important step for preparing alpha-synuclein PFFs for use in vitro and in vivo. 22 Preserving Endogenous Fluorescent Protein Signals for Volumetric Imaging Using the X-CLARITY Systems and Reagents for Tissue Clearing Step-by-step protocol describes how to obtain images of endogenous FP signals from transparent mouse brains. 2