eBook: Advancing Neuroscience Research - 9

Advances in tissue clearing
Advances in tissue clearing are paving the way for
more light sheet microscopy studies. " There is no
limit to the type of tissue you can image with light
sheet microscopy, as long as it is transparent, " explains
Monika Löschinger, Senior Product and Applications
Manager at Luxendo. " With clearing techniques
and light sheet microscopy, you can image
an entire clear mouse without damaging the tissue
by sectioning it, so you get a realistic 3D result for
the 3D microstructure analysis of the tissues. " She
adds that this is particularly useful in studying
brain tissue, analyzing organ development, and investigating
the formation and structure of tumors.
Light sheet microscopy is well-suited to fast, large
volume, 3D imaging in cleared whole mouse
brains or other large tissues, adds Michael Johnson,
CEO and Co-Founder of Visikol. " It is ideal for
use with large tissues that are labeled with fluorescent
proteins or small chemical dyes, as it is
very challenging to get antibodies to penetrate
deep into tissue, " he says. " Some researchers have
been able to achieve deep antibody penetration,
but it is quite challenging and tends to be expensive
and require significant method development
and validation effort. "
The final chapter in this eBook describes a protocol
to obtain images of endogenous fluorescent
protein signals from transparent mouse brains using
X-CLARITY™ systems and reagents for tissue clearing.
References
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eaat7715 (2018).
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pathology in Parkinson's disease consists of crowded
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