eBook: Breakthroughs and Innovations in Immunology Research - 10

Coating Type
Thin Layer
Coating
Thick Layer
Coating
Table 1. Coating methodologies and their applications.
Description
A thin layer of Matrigengel
Matrix is formed at the
bottom of a well plate.
A thick layer of Matrigengel
Matrix is formed at the
bottom of a well plate.
Application
* iPSC expansion
* Tumor Cell
Invasion Assays
* Tumor Disease
Modeling
* Organoid Culturing
* Angiogenesis
Modeling/Tube
Formation
Sandwich
Coating
Embedded
Dome Coating
Two thick layers of Matrigengel
Matrix are formed to surround
the cultured cells.
Cells are mixed with liquidform
Matrigengel Matrix and
plated in a dome-formation.
* iPSC Differentiation
* Organoid Culture
a successful model, as well as quality and consistency.
At ACROBiosystems, we are currently partnering
with Mogengel Bio through Acro Certify to bring
you an application-validated basement membrane
extract, Matrigengel Matrix. For every batch manufactured,
application studies are performed, such
as organoid culture, stem cell culture, angiogenesis,
tumor invasion, and tumor xenografts, to ensure
that our products can support the optimal application
requirements.
References
permeability, and adhesivity. As such, this can significantly
affect its performance regarding the desired
application, especially with harder-to-culture
cells that have more specific requirements for cell
growth. For a quick overview of the coating type
and desired application, please refer to Table 1.
Conclusion
Overall, basement membrane extracts are a versatile
reagent that is critical to both basic and
translational research. Starting from cell culturing
and all the way to disease modeling, basement
membranes are a critical component toward understanding
and interpreting the interplay between
molecules, cells, and tissue layers, especially
in immunology and disease pathology. Using a
more natural-like basement membrane extract in
comparison to synthetic extracts in general gives
better results. However, in comparison to synthetic
derivatives, basement membrane extracts are
prone to batch-to-batch diversity and can be hard
to work with.
Tailoring the right concentration and protocol for
any application is always critical toward developing
1. Arnaoutova, I., George, J., Kleinman, H. K. & Benton,
G. Basement Membrane Matrix (BME) has Multiple
Uses with Stem Cells. Stem Cell Rev. Reports 8,
163-169 (2012).
2. Benton, G., Kleinman, H. K., George, J. & Arnaoutova,
I. Multiple uses of basement membrane-like matrix
(BME/Matrigel) in vitro and in vivo with cancer cells.
Int. J. Cancer 128, 1751-1757 (2011).
3. Aisenbrey, E. A. & Murphy, W. L. Synthetic alternatives
to Matrigel. Nature Reviews Materials vol. 5 (2020).
4. Youn, J. & Kim, D. S. Engineering porous membranes
mimicking in vivo basement membrane for organ-onchips
applications. Biomicrofluidics 16, 1-7 (2022).
5. Lopera Higuita, M., Shortreed, N. A., Dasari, S.
& Griffiths, L. G. Basement Membrane of Tissue
Engineered Extracellular Matrix Scaffolds Modulates
Rapid Human Endothelial Cell Recellularization
and Promote Quiescent Behavior After Monolayer
Formation. Front. Bioeng. Biotechnol. 10, 1-15 (2022).
Additional Resource
Matrigengel Matrix:
Basement Membrane Extracts
10

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