eBook: Cell and Gene Therapy - 15

reporter cells.
Importantly, cytotoxicity in these
luciferase-expressing cells could be measured by
the loss of BLI signal in real time, and this loss was
confirmed by live cell imaging and cytotoxic dye
uptake assays. In summary, the well-characterized
luciferase reporter cell lines enable convenient
and consistent signal quantification, and they are
easy-to-use tools for studying CAR-T biofunction
and validating new CAR-T agents for cancer immunotherapy.
These robust cell models are representative
of the most predominant patient-derived
carcinoma and lymphoma cancer lines used
in oncology research. The CAR-T Target Luciferase
Reporter Cells were selected from ATCC's extensive
catalog of established cancer cell lines that contain
high endogenous expression of some of the most
prevalent cancer antigens, which makes them
more physiologically relevant as in vitro tools to develop
adoptive CAR-T cell therapies.
References
1. Kiesgen S, et al. Comparative analysis of assays to
measure CART-cell-mediated cytotoxicity. Nat Protoc
16:1331-1342, 2021. PubMed: 33589826
2. Jackson HJ, et al. Driving CAR T-cells forward. Nat Rev
Clin Oncol 13: 370-383, 2016. PubMed: 27000958
3. Sterner RC, Sterner RM. CAR-T cell therapy: current
limitations and potential strategies. Blood Cancer J 11:
69, 2021. PubMed: 33824268
4. RissT,etal. Cytotoxicity Assays: In Vitro Methods to
Measure Dead Cells, in Assay Guidance Manual. (eds.
S. Markossian et al.) (Bethesda (MD); 2004). PubMed:
31070879
5. Meijer TWH, et al. Targeting glucose and glutamine
metabolism combined with radiation therapy in nonsmall
cell lung cancer. Lung Cancer 126: 32-40, 2018.
PubMed: 30527190
6. Lisby AN, et al. Evaluation of CAR-T cell cytotoxicity:
Real-time impedance-based analysis.Methods Cell
Biol 167: 81-98, 2022. PubMed: 35153000
7. Capes-Davis A, et al. Check your cultures! A list of
cross-contaminated or misidentified cell lines. Int J
Cancer 127: 1-8 2010. PubMed: 20143388
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