Figure 1. Molecular characterization of 3 VHG cell lines. (A - C) Sanger sequencing of the STAT1 gene of all 3 virus-producing KO cells confirms the disruption of the STAT1 gene. (D) Sanger sequencing of the BAX gene of 293.STAT1 BAX KO cells confirms BAX gene disruption. (E-G) STAT1 and BAX immunoblot of total cellular protein from parental wild type and knockout cells confirms absence of both STAT1 protein or STAT1 and BAX protein expression in the enhanced virus-producing cell lines. 19