eBook: Cell and Gene Therapy - 25

Table 1. Observed total mean sample copy number relative to predicted input copy number measured by ddPCR and QPCR (N=2, n=6).
number when using the QPCR platform. These results
indicate that both molecular-based detection
systems are able to sensitively detect and quantify
mycoplasma DNA, with the QPCR platform exhibiting
more accuracy with lower concentrations of
genomic material.
Conclusion
The use of quantitative external DNA controls provides
an effective approach for the development
and evaluation of novel molecular-based assays
for the detection of mycoplasma contamination.
Quantitative mycoplasma DNA controls are currently
available as certified reference materials
from ATCC under the catalog numbers qCRM15531D™,
qCRM-17981D™, qCRM-19610D™,
qCRM-19989D™, qCRM-23064D™, qCRM-23206D™,
qCRM-23714D™, qCRM-23838D™, qCRM-25204D™,
and qCRM-27545D™.
References
1. Drexler H.G., and Uphoff C.C. Mycoplasma
contamination of cell cultures: Incidence, sources,
effects, detection, elimination, prevention.
Cytotechnology 39, 75-90 (2002).
2. McGarrity G.J., Constantopoulos G., and Barranger
J.A. Effect of mycoplasma infection on pyruvate
dehydrogenase complex activity of normal and
pyruvate dehydrogenase complex-deficient
fibroblasts. Exp Cell Res. 151, 557-562 (1984).
3. Rottern S. Interaction of Mycoplasma with Host Cells.
Physiol Rev 83, 15 (2003).
4. Chandler D.K.F, Volokhov D.V., Chizhikov V.E.
Historical overview of mycoplasma testing for
production of biologics. Am Pharm Rev. http://www.
americanpharmaceuticalreview.com/FeaturedArticles/37370-Historical-Overview-of-MycoplasmaTesting-for-
Production-of-Biologics/ (2011).
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