eBook: Cell and Gene Therapy - 27

life-saving protein can be commercially produced
in the bacterium Escherichia coli through the incorporation
of the human insulin gene into the bacterial
genome via recombinant DNA technology.2,3
Once the gene is in place, insulin is produced using
normal cellular machinery and is then removed
and purified as a medicine for the millions of people
living with diabetes.
What's needed?
The performance of the bioprocess and the quality
of the produced biologic can be influenced by the
starting materials used. For these critical bioproduction
products, it is important to have access to:
* High-quality, authenticated biological
materials sourced from an established
biorepository
* Curated reference-quality 'omics data that
are traceable to credible materials
*
A facility equipped for the manufacture and
storage of both the starting materials and
final products
How we can help
ATCC supports bioproduction research and development
efforts by providing the scientific community
with access to an extensive portfolio credible
resources such as Vero, MDCK, and HEK-293 cell
lines-including STAT1 and BAX knockout cell lines
for enhanced virus production. We also offer purified
pneumococcal polysaccharides that can be
used in vaccine development and microbial strains
with potential applications in antibiotic, organic
acid, and biofuel production.
2. Baeshen NA, et al. Cell factories for insulin production.
Microb Cell Fact 13: 141, 2014. PubMed: 25270715
3. Zieliński M, et al. Expression and purification of
recombinant human insulin from E. coli 20 strain.
Protein Expr Purif 157: 63-69, 2019. PubMed:
30735706
4. U.S. Food & Drug Administration. Coronavirus Disease
2019 (COVID-19) Emergency Use Authorizations for
Medical Devices. Accessed online https://www.fda.
gov/medical-devices/emergency-use-authorizationsmedical-devices/coronavirus-disease-2019-covid-19emergency-use-authorizations-medical-devices.
5.
Baeshen NA, et al. Cell factories for insulin production.
Microb Cell Fact 13: 141, 2014. PubMed: 25270715
6. Zieliński M, et al. Expression and purification of
recombinant human insulin from E. coli 20 strain.
Protein Expr Purif 157: 63-69, 2019. PubMed:
30735706
About the Authors
Brian Shapiro, Ph.D., and Cara Wilder, Ph.D., are
with ATCC.
References
1. U.S. Food & Drug Administration. Coronavirus Disease
2019 (COVID-19) Emergency Use Authorizations for
Medical Devices. Accessed online https://www.fda.
gov/medical-devices/emergency-use-authorizationsmedical-devices/coronavirus-disease-2019-covid-19emergency-use-authorizations-medical-devices.
27

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