eBook: New Frontiers in Infectious Disease Research - 22

1 ng) resulting from host depleted samples. Within
2.5 hours, Unison shortens the hands-on time
and total sequencing library preparation time.
Through the streamlining of the required sequencing
depth, NGS becomes more affordable and a
higher number of samples can be processed using
the same volume of reagents. Using the Devin filter
to enrich microbial DNA optimizes cost, as it will
allow multiplexing of each sequencing run, which
can reduce sequencing costs up to 5-fold.
Figure 4. Results of human and microbial identification
using qPCR, NGS, and Nanopore
DNA-based mNGS, showing an average reads-permillion
(RPM) of 2,359 compared to only 95 by a
cfDNA-based method.10
The importance of host depletion in
reducing sequencing costs
Despite the increasing affordability of sequencing
per megabase of DNA, sequencing still represents
a major portion of the total costs associated with
NGS-based pipelines. However, by effectively eliminating
host contamination, the sequencing output
required to achieve a representative microbiome
sample can be significantly reduced, which in
turn, decreases costs or significantly shortens the
sequencing time, depending on the downstream
sequencing platform.
Micronbrane Medical combines the Devin filter
with a gDNA based workflow, the PaRTI-Seq assay,
to offer research, translational, and clinical laboratories
a fast, efficient, and affordable mNGS-optimized
solution. Micronbrane Medical's Unison
Ultralow DNA NGS Library Preparation Kit is specifically
optimized for ultralow DNA input (10 pg ~
Lastly, NGS generates a significant amount of raw
data that can be complex to handle, as it requires
sophisticated bioinformatics software, computational
hardware and specialized human resources.
All these factors entail several layers of logistical
and financial constraints that need to be accounted
for to accelerate the integration of NGS-based
pipelines in molecular laboratories.
Micronbrane Medical's PaRTI-Cular™ is a bioinformatics
one-click install software, deeply curated
with up-to-date microorganism genome information,
proper benchmarking and analysis of microbial
pathogens with unusual sequence homologies
and/or close taxonomic relationships to decrease
false-positive or false-negative results. PaRTI-Cular's
state-of-the-art computational algorithms are
fast and provide a report in 30 minutes per sample.
The cloud-based software also automatically
conducts data quality checks, removal of host genome
reads, pathogen identification and statistical
results analysis.
Innovating metagenomics
Micronbrane Medical's Devin filter and PaRTI-Seq
assay helps to overcome the barriers to widespread
adoption of mNGS with a single test that
more comprehensively profiles samples. The
combined solution enables fast and accurate
22
eBook: New Frontiers in Infectious Disease Research

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