eBook: Optimizing Cell Culture Techniques - 13

4. Biliary canaliculi activity of HepG2
Biliary canaliculi activity of HepG2 cells treated
with chlorpromazine was examined using 5(6)-carboxy-2',7'-dichlorofluorescein
diacetate (CDFDA)
(21884, Sigma)
Remove 100 μl of culture medium and add 200 μl
of PBS to the HepG2 cells, using the normal Pipet
mode with the same Z-height and speed described
in step 2. Wash the HepG2 cells by adding and removing
200 μl of PBS (repeat this step five times).
The convenient three position stage of the VIAFLO
96 and VIAFLO 384 accommodates the waste and
reagent reservoirs (positions A and AB, respectively)
needed for the washing step, and a target sample
plate (position B).
Conclusion
* Easy liquid handling, time savings and high
accuracy in generating identical data sets.
* Very low variability in cellular metabolic
activity between wells.
* Treatment, seeding and viability assays are all
performed with the VIAFLO 96 and VIAFLO 384.
* Easy observation using a microscope.
Additional Resource
Pipetting solutions for all your
cell culture needs
13

https://www.integra-biosciences.com/en/home-pipetting-solutions-all-your-cell-culture-needs

eBook: Optimizing Cell Culture Techniques

Table of Contents for the Digital Edition of eBook: Optimizing Cell Culture Techniques