CES - Revista Veterinaria Julio - Diciembre 2010 - (Page 18)

Purification, characterization and analysis of sepia melanin from commercial sepia ink (Sepia Officinalis) Purificación, caracterización y análisis de la melanina de sepia a partir de la tinta de sepia (Sepia Officinalis) Mario Magarelli1*, Químico, cPhD; Paolo Passamonti1, Químico; Carlo Renieri1, Biólogo. 1 Department of Environment Sciences, Chemical section, University of Camerino, Via S. Agostino 1 Camerino 62032, Italy * Corresponding author: Universidad of Camerino, Department of Environment Sciences, Chemical section, Via S. Agostino 1 Camerino 62032, Italy. E-mail: mario.magarelli@unicam.it (Recibido: 07 de septiembre, 2010; aceptado: 17 de noviembre, 2010) Abstract At the moment melanins are still enigmatic bio-pigments with structure and determination methods not clearly understood for the international scientific community. Among biopolymers, melanins are unique in many aspects. Some essential biopolymers (proteins, nucleic acids and carbohydrates) are chemically well characterized and can be determined using well established analytical methodologies. On the contrary, no available methods allow us to accurately determine melanin, mostly due to the intrinsic chemical properties of melanins. These pigments, in fact, are insoluble in a broad range of solvents and pH as well as difficult to purify as a result of the heterogeneity in their structural features. Mammalian melanins exist in two chemically distinct forms: the brown to black Eumelanin and the yellow to reddish-brown Pheomelanin. Sepia melanin obtained from Sepia Officinalis consists of more than 98% of Eumelanin and is therefore used as standard material in the analysis of melanic black. Commercial sepia melanin is purified according to an undescribed procedure. In this research, extraction and purification studies were carried out on sepia melanin using a hydrochloric acid (0.5 - 3.0 M) treatment under mechanical or ultrasonic agitation. A high degree purity sepia melanin was obtained and further characterized using Elemental Analysis (EA), UltravioletVisible (UV-VIS) and Infrared (IR) spectroscopy and Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) for metal ion analysis. Finally, quantitative studies of the chemical degradation of Eu polymers to Pyrrole-2,3,5tricarboxylic acid (PTCA) and Pyrrole-2,3-dicarboxylic acid (PDCA) by High Performance Liquid Chromatography (HPLC) were performed. The procedure we developed can be used to obtain a considerably less expensive sepia melanin which could prove important as a standard in future determinations of eumelanin and pheomelanin as well as in a vast field of scientific and industrial applications in such fields as human and veterinary medicine, pharmacology and cosmetics. Key words Characterization, eumelanin, purification, sepia melanin. *To cite this paper: Magarelli M, Passamonti P, Renieri C. 2010. Purification, characterization and analysis of sepia melanin from commercial sepia ink (Sepia Officinalis). Rev CES Med Vet Zootec; Vol 5 (2): 18-28 18 Revista CES Medicina Veterinaria y Zootecnia / Volumen 5 / Número 2 / Julio – Diciembre de 2010 / ISSN 1900-9607 http://www.revistamvzces.com/revistas/vol6no1/Articulo_2.pdf

Tabla de contenidos de la publicati�n de CES - Revista Veterinaria Julio - Diciembre 2010

CES - Revista Veterinaria Julio - Diciembre 2010
Contenido
Inoculation with Mycobacterium plhei inhibits allergic inflammation in a rabbit model of ovalbumin (ova) sensitization
Purification, characterization and analysis of sepia melanin from commercial sepia ink (Sepia Officinalis)
Estimación de las curvas de lactancia y producción de leche de cabras del departamento de Antioquia, usando controles lecheros quincenales y mensuales
Evaluación histoquímica preliminar para determinar la presencia de algunos glucocomponentes de la mucosa de las bolsas guturales de los equinos
Evaluación del suministro de un alimento especial en el transporte y dos primeros días de vida del pollo de engorde y los beneficios sobre su desempeño zootécnico y económico
Medición de las enzimas AST y GGT en diferentes estados reproductivos y/o edades en caballo Criollo Colombiano en el Valle de Aburrá, Antioquia
Análisis retrospectivo de los registros clínicos del Centro de Veterinaria y Zootecnia de la Universidad CES 2004-2009
Emergencias gastrointestinales en perros y gatos
Corrección quirúrgica de un atrapamiento del intestino delgado en el foramen epiploico en un caballo Pura Sangre Inglés (PSI)

CES - Revista Veterinaria Julio - Diciembre 2010