The Column - March 2007 - (Page 30)
Q&A The Column www.thecolumn.eu.com March 2007 The Beginning of a New Age? Gel electrophoresis (GE) has been the academic scientists’ separation technology of choice to analyse proteins since the 1970s. The Column spoke to deltaDOT co-founder Dr Stuart Hassard about why he believes this technology is obsolete and why the future for protein analysis — and many other problems encountered by separation scientists — is label-free intrinsic imaging (LFII) connected to capillary electrophoresis (CE). deltaDOT was set up to develop innovative ways to separate and analyse biomolecules. Why are you entering this market? The company was formed in 2000 as a spin-out from Imperial College London and is the result of a unique collaboration of two very disparate disciplines. I’m a molecular biologist and my co-founder and older brother, John, is a particle physicist. Together, we had the innovative idea of taking signal processing and multiple detector techniques from particle physics and applying them to modern biomolecular analysis. One of the early fruits of our collaboration was a novel biomolecule Contact: Stuart Hassard E-mail: s.hassard@deltadot.com detection technique, label-free intrinsic imaging (LFII), which allows the separation and analysis of amino acids, peptides, proteins, deoxyribonucleic acid (DNA) fragments and nucleic acids. Coupled with proprietary algorithms and analytical methods, LFII forms the basis of the core technology in our pipeline of high performance capillary electrophoresis (HPCE) products. When capillary electrophoresis (CE) was first introduced in the 1990s for the separation and analysis of biomolecules, the technology was taken up by large pharmaceutical companies. However, it failed to gain acceptance in academic laboratories. Protein analysis techniques using polyacrylamide gels have been the technology of choice in university labs since the 1970s but have changed very little since their inception. Nucleic acid separation techniques that also use gels and require labelling are available too, but protein analysis and nucleic acid separation techniques have various technical drawbacks, including slow run-time and low resolution. So we are going into a market that is latent and we also think that the gel system is now old technology whose time has come to an end. Most academics don’t know that CE even exists and they perceive that the comfortable old gel system is their only choice — like owning a second-hand car when you could have an Aston Martin. 30
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Table of Contents for the Digital Edition of The Column - March 2007
Contents
News
Zosimus
Market Trends and Analysis
Radioactive Chromatography: What is the Smallest Detectable Peak?
Automated Analysis and Data Evaluation of Explosives' Residues
Q&A
The Column - March 2007
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