completely new approach. Here the mobile phase remains constant and
optimization of the stationary phase is the primary goal. POPLCTM is based
on the theory of the Prisma model that has been used before to optimize
mobile phases mainly in thin layer www.poplc.de chromatography TLC and
normal phase HPLC [1]. The Prisma is shown in Fig. 1. Prisma Model A New
and Effective Tool to Optimize HPLC Methods! Introduction Optimization of
Liquid Chromatography Separations computer software is used to optimize
the mobile has been done with several techniques in the past. phase and
therefore the separation. Those Some scientists prefer to keep the
stationary phase software support is very helpful in some cases and
constant and do the optimization mainly by changing saves a lot of time of
trial and error in comparison to pH, type of organic modifier methanol or
acetonitrile manual procedures. Never the less it will not help to or the
temperature of the mobile phase. Others first resolve two components if
those can not be apply a standard gradient on several different types of
separated on the stationary phase which is used for The vertical sides of
the prism correspond to the stationary phases and use the best suited
column for the corresponding separation. retention strength of a given
stationary phase with further optimization of mobile phase. There are also
In Phase OPtimized Liquid Chromatography regard to a given analyte. The
optimal retention some optimization procedures existing where POPLCTM the
optimization procedure follows a strength for this analyte is somewhere on
the surface that is built by the upper irregular triangle of the prism.
http://www.poplc.dehttp://www.poplc.de
Table of Contents for the Digital Edition of The Column - September 2006