eBook: TOC and Microbial Detection Monitoring - 38

Case Study

Bioburden Analysis for Process Control
Reduces Plate Counting Costs and Delays

A major influenza vaccine manufacturer in the US was concerned with intermittent positive plate
counts on its water system. These results led to more frequent offline testing to continue to
monitor the water system and find the root cause. However, this method did not provide enough
process transparency to make operational decisions. The customer turned to the 7000RMS™
analyzer for continuous bioburden monitoring to get better insight into their process while
reducing the costs of multiple plate countings and product delays.
Repeated plate counts led to delayed
product release
During the pharmaceutical manufacturing process, one of the biggest challenges is achieving
timely product release while ensuring the product is safe for consumers. A major bottleneck
in manufacturing is microbial contamination
testing of the process water to ensure it does
not contaminate the final product. The traditional
method of microbial tests - plate counting -
takes five to seven days to give a result, during
which time the product must be quarantined.

This method only provides a snapshot in time
of the water loop, potentially missing outof-specification events. Moreover, if a plate
count produces a positive result, the test is
usually repeated to confirm the contamination
and then further investigation is required
leading to more delays.
With these process delays in mind, the customer
started to explore ways to reduce the time to
product release while also controlling process
costs. As they investigated ways to streamline



eBook: TOC and Microbial Detection Monitoring

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