Figure1. Experimental design to compare single-cell and bulk sequencing. The diagram shows a workflow for comparing unstimulated and Th1 polarized human PBMCs using BEN-seq or single-cell proteogenomics. Figure 2. Polarized Th1 cells show expected cytokine profile. Flow cytometry analysis of Th1-polarized PBMCs shows production of classical Th1-associated cytokines (dot plots, top panels). Secreted levels of cytokines were measured using BioLegend's Th1 LEGENDplex™ panel, showing strong correlation of high IL-2, IFN-γ, and TNF-α levels, as well as low levels of Th2 phenotype cytokines like IL-4, IL-5, and IL-10. 16