Figure 1. Examples of recombinant checkpoint proteins from Sino Biological. (A) The purity of recombinant human CD47 his tag (Cat: 12283-H08H) protein is greater than 95 % as determined by SDS-PAGE. (B) The purity of recombinant human LAG3 tag free protein (Cat: HPLC-16498-HNAH) is greater than 95 % as determined by SEC-HPLC. (C) Bioactivity assay of recombinant human CD47 his tag protein. Immobilized Human SIRP alpha hFc (Cat: 11612-H02H1) at 2 μg/ml (100 μl/well) can bind Human CD47 His (Cat: 12283-H08H). Figure 2. Examples of biotinylated and unconjugated proteins from Sino Biological. (A) Bioactivity assay of biotinylated recombinant human TIGIT Fc tag protein (Cat: 10917-H02H-B). Immobilized human CD155 hFc tag protein Cat: 10109-H02H) at 2 μg/mL (100 μL/ well) can bind Biotinylated human TIGIT hFc tag (Cat: 10917-H02H-B). (B) Recombinant human CTLA-4 His tag protein (Cat: 11159H08H) inhibits IL-2 secretion by stimulated Jurkat cells. tor-ligand pathway. For example, therapeutic antibodies blocking PD-1 and its ligand PD-L1 have already shown clinical efficacy.8 And Anti-CD47 antibodies enhance the phagocytosis of tumor cells by macrophages by blocking the binding of CD47 to SIRPα.9 pair proteins (Figure 3) together. So we usually develop ligand-receptor We also offer Cynomolgus, Rhesus, Mouse, and Rat proteins (Figure 4) for cross-reactivity and animal studies. Cytokine related products In the development of antibodies, the functional activities of antibodies need to be tested on cells. We offer cytokine-related products for cell-based func8