eBook: Multiplex Technology Guide - 15

Table 2. Limit of Detection, Lower Limit of Quantification, and Dynamic Range of 16 overlapping cytokine analytes
measured by the bead-based fluorescence (LMX) and planar electrochemiluminescence (MSD) multiplex immunoassays.
LMX

MSD

16 Shared Analytes

LoD* (pg/mL)

LLoQ* (pg/mL)

Dynamic Range (pg/mL) LoD * (pg/mL)

LLoQ* (pg/mL)

Dynamic Range (pg/mL)

GM-CSF

5.91

5.91

5.91-20,000

0.38

1.05

1.05-4,280

IFN-γ

2.01

2.44

2.44-10,000

1.43

1.43

1.43-5,080

IL-1β

0.61

1.95

1.95-8,000

0.10

0.56

0.56-2,296

IL-2

2.41

2.41

2.41-8,000

0.22

1.37

1.37-5,600

IL-4

8.21

8.21

8.21-30,000

0.03

0.21

0.21-872

IL-5

0.88

1.95

1.95-8,000

0.42

0.81

0.81-3,320

IL-6

0.59

0.73

0.73-3,000

0.09

0.73

0.73-2,992

IL-7

1.16

1.46

1.46-6,000

0.28

0.83

0.83-3,384

IL-8

0.78

1.22

1.22-5,000

0.08

0.54

0.54-2,212

IL-10

3.77

5.86

5.86-24,000

0.08

0.37

0.37-1,504

IL-12 (p70)

0.71

1.95

1.95-8,000

0.10

0.54

0.54-2,204

IL-13

2.19

2.19

2.19-4,000

0.41

0.49

0.49-2,012

IL-17

1.63

2.93

2.93-12,000

0.87

6.09

6.09-24,960

MIP-1α

18.90

18.90

18.90-5,000

7.25

7.25

7.25-4,560

MIP-1β

7.09

7.09

7.09-15,000

0.90

1.15

1.15-4,720

TNF-α

0.63

1.71

1.71-7,000

0.16

0.32

0.32-1,292

*LoD = Limit of Detection, LLoQ = Lower Limit of Quantitation.

Table 3. Percentage of human plasma samples with target cytokines below the LLoD using the Luminex (LMX) and Meso
Scale Discovery (MSD) multiplex assays. Bolded values signify that the target cytokine was undetected (below LLoD) in
≥50% of samples by the indicated platform.
Shared Analyte

LMNX-Only % Missed

MSD-Only % Missed

LMNX and MSD % Missed

GM-CSF

5.4

56.8

5.4

IFN-γ

9.7

6.5

1.6

IL-1β

4.8

56.5

19.4

IL-2

3.2

62.9

17.7

IL-4

6.5

71.0

6.5

IL-5

21.6

5.4

0.0

IL-6

1.6

51.6

4.8

IL-7

8.1

0.0

0.0

IL-8

0.0

0.0

0.0

IL-10

6.5

17.7

14.5

IL-12 (p70)

6.5

53.2

14.4

IL-13

22.6

25.8

4.8

IL-17

10.8

0.0

0.0

MIP-1α

54.1

0.0

0.0

MIP-1β

2.7

0.0

0.0

TNF-α

0.0

0.0

0.0

15
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