eBook: Multiplex Technology Guide - 16

Figure 1. Dynamic ranges of the Luminex
bead-based fluorescence (LMX) and Meso
Scale Discovery electrochemiluminescence
(MSD) multiplex cytokine immunoassay
kits. With some exceptions (e.g., IL-6 and
IL-17), the low end of a shared analyte's dynamic range was lower with the MSD compared to the LMX assay. Conversely, and
with some exceptions (e.g., IL-6, IL-17, and
MIP 1α), the high end of the dynamic range
was greater with the LMX assay vs. the MSD
assay. In two instances (IL-4 and IL-10), the
differences in upper and lower quantification levels differed by at least an order of
magnitude between the two assays.

Figure 2. Proportion of donor plasma samples outside the assay quantification range for the Luminex (LMX) and Meso Scale Discovery
(MSD) multiplex cytokine immunoassay kits. Although the MSD assay had a LLoQ below that of the LMX assay for 14/16 common analytes in earlier standard curve performance evaluations, this did not directly translate to evaluation of cytokines in experimental human
plasma samples, whereby several analytes were missed at a greater frequency with the MSD platform (e.g., IL-10, IL-12, IL-1β, IL-2, IL-4,
IL-6, GM-CSF), and others were missed at a greater frequency with the LMX platform, but detected with the MSD assay (e.g., IFN-γ, IL-5,
IL-7, IL-17, MIP 1α, MIP-1β).

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