eBook: Multiplex Technology Guide - 24

Guidelines for Developing Multiplex
SARS-CoV-2 Serology Assays
In a recent addendum to the xMAP® Cookbook, learn how to build
rapid-result serology assays to help manage the COVID-19 outbreak.
Since SARS-CoV-2 emerged in late 2019, single-target serological assays have played a critical role in
detecting viral antibodies induced by prior infection.
However, single-target assays have some limitations,
especially if there are varying levels of antibodies to
different parts of the virus in recovered patients.

Highlights of the addendum include:

Bead-based multiplex technology is ideal for developing serological assays, especially because it can
detect antibodies to more than one viral antigen at a
time. It's clear why demand is surging for these tests-
many xMAP® assays have already been developed
and adapted to detect several antibodies associated
with the SARS-CoV-2 virus in labs across the world.

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A review of relevant structural proteins

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Protein acquisition guidance

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Considerations for coupling antigens to
xMAP® beads

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Supply lists specific to SARS-CoV-2 serological
assay development

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A step-by-step development guide

Combined with the complete xMAP Cookbook and
the support of Luminex's Field Applications Team,
this addendum will help assay developers of all experience levels get started quickly and build novel,
rapid-result xMAP serological assays to help manage
the evolving COVID-19 outbreak.

Given the current state of the global pandemic, assay developers are eager to contribute to the clinical research community. To help familiarize scientists with the process of developing bead-based
assays, particularly as they relate to the SARS-CoV-2
virus, the Luminex Team has developed an addendum to the latest version of the xMAP Cookbook,
titled " Planning SARS-CoV-2 Serological Assay
Development. " This addition can help guide users in
key considerations and decisions that are critical to
optimal assay performance.

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eBook: Multiplex Technology Guide

Table of Contents for the Digital Edition of eBook: Multiplex Technology Guide
