eBook: Multiplex Technology Guide - 28

antibodies, and to monitor non-specific binding and
instrument performance. Any data outside of an acceptable range can then serve as strong justification
for removing a single well from a data set before reporting results.

provide this standard of quality. For a semi-quantitative assay, a reasonable strategy is to use an external control for each category measured in the assay.
For quantitative assays, it's ideal to include high, low,
and negative controls spanning the range of the
standard curve to confirm data quality.

If you'd like more information about Luminex's
growing repertoire of open-source software analysis
tools, reach out to a Field Applications Scientist for
the most updated list of resources.

Internal controls are a special feature of the multiplexing capabilities of xMAP® Technology. They
undergo exactly the same treatment in each well
as the other analyte beads in the assay, and can be
designed to control for a number of assay-influencing events. These beads can be used to check for
the addition of a fluorescent reporter, detection of

About the author
Heather Darby is a Field Applications Scientist at
Luminex.

28

https://www.luminexcorp.com/luminex-field-application-support/

eBook: Multiplex Technology Guide

Table of Contents for the Digital Edition of eBook: Multiplex Technology Guide