eBook: Multiplex Technology Guide - 6

3D), where one laser (635 nm) classifies the bead and
the other (532 nm) determines the magnitude of the
PE-derived signal. As an alternative to flow-based
detection, magnetic MagPlex® beads can be read
using the Luminex MAGPIX® System.

COVID-19. Further uses for the xMAP® INTELLIFLEX
include free versus bound drug testing, detection
of protein cleavage and splice variants, and genomic applications such as SNP genotyping and
evaluation of gene expression.

The xMAP® INTELLIFLEX System is the latest addition
to the xMAP® family, designed to take Luminex' technology to new levels. Featuring a second reporter laser (405 nm) alongside the traditional reporter channel, it enables the detection of two parameters on a
single bead, doubling the data for up to 500 analytes
per well. This equates to decreased sample volumes
and the potential for an even greater number of
readouts than before, as well as enhances the scope
of biological assays that are currently performed using existing xMAP® technologies.

With an enhanced dynamic range of up to 5.5 logs
and a compact footprint enabled by an integrated
touchscreen computer to reduce bench space, the
xMAP® INTELLIFLEX System builds on current multiplexing technologies, giving researchers even greater choice when it comes to selecting a platform that
best fits their requirements. Surely now the question
isn't 'why multiplex?' but rather 'which multiplexing
platform should I use?'

About the author

For example, on a single bead, researchers can
now compare total and phosphorylated forms of
a protein or monitor two distinct post-translational modifications. It is also possible to use the same
bead for measuring two antigen-specific antibody
isotypes, an approach that has received significant interest from researchers wishing to perform
serology assays to study the immune response to

Emma Easthope is the founder and director of
Cambridge Technical Content Ltd. Since graduating
with a bachelor's degree in biology from the University
of Kent at Canterbury in 2000, she has gained extensive experience developing and running immunoassays within companies including Millennium
Pharmaceuticals, AstraZeneca, and Cellzome.

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eBook: Multiplex Technology Guide

Table of Contents for the Digital Edition of eBook: Multiplex Technology Guide
