Neurogenerative Diseases - 13

Target protein

Cat. No.

HSP40

PA5-17382

HSP60 (clone 4B9/89)

MA3-012, PA5-34760

HSP60

PA5-34760

HSP70 (clone 4G4)

MA3-009

HSP70 (clone 2A4)

MA3-008

HSP90 alpha (clone 3B6)

MA3-010

HSP90 beta (clone H9010)

37-9400

Phospho-HSP27 (Ser15)

PA1-018

Phospho-HSP27 (Ser85)

PA1-005

Table 1. Suggested Antibodies Against Heat-Shock Proteins (HSP)
Implicated in Parkinson's Disease.

Figure 2. Assessment of alpha-synuclein antibody specificity. (A) Immunofluorescence analysis in mouse brain using alpha-synuclein
polyclonal antibody (red, Cat. No. PA5-17239) and a neurofilament-L
monoclonal antibody (green); DNA is labeled using DAPI. Western
blot analysis of alpha-synuclein antibody using (B) CRISPR knockout (KO) of alpha-synuclein in HeLa cells and (C) relative expression
of alpha-synuclein in brain and liver. Western blot detection was
performed as described in Figure 1.

Figure 3. Assessment of antibody specificity. Western blot analysis of
(A) Parkin (clone 21H24L9, Cat. No. 702785) and (B) DJ-1 (Cat. No. PA513404) antibodies to show specificity using CRISPR knockout (KO)
and RNAi, respectively. (C) Immunofluorescence analysis in HepG2
cells labeled with LIMP2 antibody (clone 22H6L14, Cat. No. 703037)
and Invitrogen™ LysoTracker™ Red DND-99 (Cat. No. L7528). (D) Immunofluorescence analysis in untransfected, scrambled, or DNAJC13
(RME8) siRNA-silenced A431 cells using DNAJC13 antibody (clone
11H13L5, Cat. No. 702773). (E) Immunofluorescence analysis of optineurin in mouse retina using optineurin oligoclonal antibody (green,
Cat. No. 711879). Western blot analysis of the optineurin antibody (F)
in liver and eye lysates and (G) in siRNA-mediated optineurin-silenced
NIH3T3 cells. Western blot detection was performed as described in
Figure 1.

bodies, and their main structural component alphasynuclein, serve as diagnostic hallmark of the final
stages of the disease, their mechanism for neuronal
degeneration is not fully understood due to a lack
of quality reagents that can help decode them at a
molecular level.9-11 Figure 2 demonstrates validation
data for the Invitrogen™ alpha-synuclein antibody,
which was tested using different parameters to
ensure its specificity.

stress, emphasizing the role of mitochondria in
neuronal degeneration and disease progression.12
Similarly, impaired lysosome-mediated protein
degradation via autophagy or endocytosis directly
impacts the ability of neurons to clear alpha-synuclein
aggregates in Lewy bodies. 13,14

A growing number of genes associated with PD
are related to mitochondrial function and oxidative

13
Neurogenerative Diseases

Table of Contents for the Digital Edition of Neurogenerative Diseases
