Origene_Jan2021_ProperAntibodyDesignKeeps - 15
Proper Antibody Design Keeps Specificity In Mind
Nanobody Approach
Prevents the Culling of Imperfect
but Still-Functional Proteins
T
o ensure the health of the cell's protein
herds, the ubiquitination system marks
misfolded or damaged proteins for
destruction. But the ubiquitination system can
be overzealous, targeting proteins that are less
than perfect, but still able to carry out their usual,
useful functions. Conceivably, if less-than-perfect
proteins were to be spared, they could serve therapeutic purposes.
This possibility is being explored by scientists
based at Columbia University. They are developing
engineered deubiquitinases (enDUBs) that can
Selvanegra / Getty Images
reverse the ubiquitination system's ruthless machinations. Importantly, the enDUBs can discriminate
between proteins that should be spared, and those
that really do need to be destroyed.
Details of this work recently appeared in Nature
Methods, in an article titled, " Targeted deubiquitination rescues distinct trafficking-deficient
Proteasomes are a large molecular machine that degrade
unneeded or damaged proteins that have been tagged
with polyubiquitin (yellow). The ubiquitin hydrolase
(violet) detaches ubiquitin from the protein that is then
unfolded and degraded into small peptides (bottom).
ion channelopathies. " This article describes how
the Columbia scientists' approach, which relies
ubiquitin chain removal from target proteins to
on a new technology that incorporates synthetic
rescue the functional expression of disparate
llama antibodies, could be used to treat dozens of
mutant ion channels that underlie long QT
diseases, including cystic fibrosis, that arise from
syndrome (LQT) and cystic fibrosis (CF), " the
the destruction of imperfect but still perfectly
article's authors wrote. " In an LQT type 1 (LQT1)
functional proteins.
cardiomyocyte model, enDUB treatment
" We developed enDUBs that enable selective
restored delayed rectifier potassium currents and
GENengnews.com
| 15
https://www.nature.com/articles/s41592-020-00992-6
https://www.nature.com/articles/s41592-020-00992-6
https://www.nature.com/articles/s41592-020-00992-6
http://www.GENengnews.com
Origene_Jan2021_ProperAntibodyDesignKeeps
Table of Contents for the Digital Edition of Origene_Jan2021_ProperAntibodyDesignKeeps
Contents
Origene_Jan2021_ProperAntibodyDesignKeeps - 1
Origene_Jan2021_ProperAntibodyDesignKeeps - 2
Origene_Jan2021_ProperAntibodyDesignKeeps - Contents
Origene_Jan2021_ProperAntibodyDesignKeeps - 4
Origene_Jan2021_ProperAntibodyDesignKeeps - 5
Origene_Jan2021_ProperAntibodyDesignKeeps - 6
Origene_Jan2021_ProperAntibodyDesignKeeps - 7
Origene_Jan2021_ProperAntibodyDesignKeeps - 8
Origene_Jan2021_ProperAntibodyDesignKeeps - 9
Origene_Jan2021_ProperAntibodyDesignKeeps - 10
Origene_Jan2021_ProperAntibodyDesignKeeps - 11
Origene_Jan2021_ProperAntibodyDesignKeeps - 12
Origene_Jan2021_ProperAntibodyDesignKeeps - 13
Origene_Jan2021_ProperAntibodyDesignKeeps - 14
Origene_Jan2021_ProperAntibodyDesignKeeps - 15
Origene_Jan2021_ProperAntibodyDesignKeeps - 16
Origene_Jan2021_ProperAntibodyDesignKeeps - 17
Origene_Jan2021_ProperAntibodyDesignKeeps - 18
Origene_Jan2021_ProperAntibodyDesignKeeps - 19
Origene_Jan2021_ProperAntibodyDesignKeeps - 20
Origene_Jan2021_ProperAntibodyDesignKeeps - 21
Origene_Jan2021_ProperAntibodyDesignKeeps - 22
Origene_Jan2021_ProperAntibodyDesignKeeps - 23
Origene_Jan2021_ProperAntibodyDesignKeeps - 24
Origene_Jan2021_ProperAntibodyDesignKeeps - 25
https://www.nxtbookmedia.com