Improved Nucleic Acid QC with Parallel CE - 3

Application Note
Quantification Practices

The Foundations of Nucleic Acid
Quality Control

Evaporation
Evaporation of a sample over time is a concern, especially during dry seasons and in drier climates. Evaporation can decrease sample
volume, changing the dilution factor, and affecting the concentration of a sample. To avoid loss of sample volume, a drop of mineral
oil can be added to the top of DNA samples in the 96-well plate protecting the samples from evaporation and retaining the ability to
prep and queue multiple runs for unattended analysis.
Summary
The Fragment Analyzer from AATI provides automated analysis of DNA smears, libraries, and fragments. Following the protocols and
best mixing practices described in each kit manual and diluting the sample with the correct buffer ensures reliable and accurate DNA
quantification.
Written by: Chava Pocernich, Ph.D.; Jolita Uthe, Ph.D.; Whitney Pike, M.A.; and Kit-Sum Wong, Ph.D
Email: techdata@aati-us.com
Kits Used in This Study:
* DNF-474 High Sensitivity NGS Fragment Analysis Kit
* DNF-487 Standard Sensitivity Genomic DNA Analysis Kit

Fragment Analyzer™ Automated CE System
The Fragment Analyzer by Advanced Analytical Technologies, Inc. offers researchers the ability
to qualify and quantify nucleic acid samples quickly and reliably through parallel capillary
electrophoresis. It was designed to solve laboratory bottlenecks and quicken the time to results
by:
*
*
*

Variable Throughput - scalable array with 12, 48, or 96 capillaries
High Separation Resolution - detection limits as low as 5 pg/µL for DNA fragments
High Sensitivity - achieve as low as 3 bp resolution

For additional application notes see our website: www.aati-us.com/application-notes
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Improved Nucleic Acid QC with Parallel CE

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