Application Note Immunophenotyping, Above and Beyond Targeting the immune system has dramatically increased the need for tracking the native and more recently engineered phenotypes of multiple cell lineages. F low cytometry has developed from being a quantitative tool for identifying and classifying mixtures of cells using fluorochrome-labeled monoclonal antibodies, to becoming an essential workhorse for cell analysis research and development. The last decade has brought a series of advancements in instrumentation and software along with an exponential expansion of antibodies and fluorochromes. This has dramatically expanded the applications and cell phenotypes that can be accurately measured with the blink of an eye1. It is no longer a characterization tool but a comprehensive phenotyping system with blazing speed and a growing rainbow of fluorophores to maximize the number of parameters that can be measured per cell. In human testing, cytometric immunophenotyping is now employed routinely to diagnose, classify, stage and monitor therapy in blood cancers and a growing list of age-related diseases.2 It can be used to determine the origin and stage of differentiation of leukemias and lymphomas.3 Sampling is nominally invasive, making it an ideal method for monitoring treatment and detecting recurrence, especially in cell-based immunotherapies.4 The deep roots and recent successes of immunotherapy in cancer treatment is rapidly changing the 21 | GENengnews.com Additional Info Read this Novocyte Quanteon application note to learn more:https://go.aceabio.com/l/492941/2018-10-22/n91jyq/492941/142853/NC_App_Note_21_Comprehensive_18_color_pan.pdf http://www.GENengnews.com