Contents A Chat with Renowned American Immunologist and Oncologist, Dr. Carl June 5 Combining HighPerformance CRISPR Guides with Sensitive, TimeResolved Cell-Based Assays 9 The Role of Potency Assays in Cell Therapy Manufacturing 15 Immunophenotyping, Above and Beyond 21 Multifunctional Potency Assays for Cancer Immunotherapy 34 25 Roundup: Quantifying Immune Cell-Mediated Killing Cover photo: Keith Chambers/Science photo Library, Getty Images