Bio-Rad eBook - 2019 - 10

Resolved "Almost
Doesn't Count"
Resolved:
"Almost
Doesn't Count"
shake

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sha k

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Yes! I can finally
see my blot!
Ummm, I really hate to
do this, but...

But? How can there
be a but? Look at this
beautiful image!!!

Did you load the
same amount of
protein in each well?

Then this won't do. Because you
can't quantitate a saturated image.

So should I shorten
the exposure times?

Yes, but let's try imaging the
membrane on my system so
we can see when it
saturates. This way we know
what we are quantifying.

Saturation or publication?
Normalization of your loading controls is the key to obtaining quantitative results. Learn how total protein
normalization using a stain-free imaging system can make the difference between quantitative blots you can
trust and blots that may not be publishable.

10

Bio-Rad eBook - 2019

Table of Contents for the Digital Edition of Bio-Rad eBook - 2019