Bio-Rad eBook - 2019 - 5

Rapid Transfer Systems
Types of Blotting Systems

Cathode (-)
Filter Paper

There are three types of transfer systems:

1. Traditional wet tank transfer systems-gel and membrane
are submerged under transfer buffer in tank
2. Semi-dry transfer systems-gel and membrane are
sandwiched between buffer-wetted filter paper in direct
contact with electrodes
3. Rapid transfer systems-use specialized materials and
buffers optimized for rapid and efficient protein transfer

Gel
Membrane
Filter Paper
Anode (+)
Gel and Membrane Setup
for Electrophoretic Transfer

Pros and Cons of Wet Tank, Semi-Dry, and Rapid Blotting Systems
Tank systems can be used for transfers of proteins of all sizes; they allow for a flexible range of power and time
settings. On the downside, they require some manual assembly and typically require cooling. Transfer times are
long-30 minutes to overnight.
Semi-dry systems are typically easier to set up than tank systems and can complete transfer in 15-60 minutes.
They don't require cooling, but power and transfer time options are limited. They perform best for proteins in the
range of 30-120 kD.
Rapid transfer systems are a more recent development. These systems utilize specialized buffers and filter papers
and come pre-wetted and sealed in single-use packages, simplifying assembly of the transfer stack. They can
be used to transfer proteins over a broad size range and don't require cooling. Rapid transfer systems are very
efficient and can complete transfer in only 3 -10 minutes.

The Trans-Blot Turbo
Rapid Blotting Transfer System
The Trans-Blot Turbo Transfer System is a highperformance western blotting transfer system designed
to provide rapid transfers with high efficiency. The system
enables blot transfer of protein in as little as 3 minutes,
with performance comparable to traditional tank transfer.
Trans-Blot Turbo Transfer Packs include an optimized
buffer, membrane, and filter paper combination that
provides superior blot transfers.

bio-rad.com/TransBlot

Six Tips for Efficient Protein Transfer
bio-rad.com/6TransferTips

https://www.bio-rad.com/transblot https://www.bio-rad.com/TransBlot http://www.bio-rad.com/6TransferTips http://www.bio-rad.com/6TransferTips http://www.bio-rad.com/6TransferTips http://www.bio-rad.com/6TransferTips

Bio-Rad eBook - 2019

Table of Contents for the Digital Edition of Bio-Rad eBook - 2019