Bio-Rad eBook - 2019 - 8

Resolved "Stuck
in the in
Matrix"
Resolved:
"Stuck
the Matrix"

Zz Z

ZzZ

There's nothing on
the membrane?!

Do you think the proteins diffused
out of the gel?...

...Or that they transferred
through the membrane entirely?

What do you
mean nothing?

Well,
there's no marker.
But I don't really
know for sure.

You should always stain
your gel and membrane
to check for the presence of
proteins. That's an important
part of optimizing
your experiment.

Where's my protein?
You need to know what's going on with the proteins both in your gel and on your blot. But how?
Stain-free gel and blot imaging.

8

Bio-Rad eBook - 2019

Table of Contents for the Digital Edition of Bio-Rad eBook - 2019