Catalent eBook - 25

Novel Approaches to Improve Cell Therapy Manufacturing

At Baylor College of Medicine's Center for Cell and Gene Therapy, the laboratory of Maksim Mamonkin, PhD, is developing
" off the shelf " allogeneic chimeric antigen receptor (CAR) T cells that will avoid host rejection upon transplantation. The
cells, which incorporate an alloimmune defense receptor (ADR), are to be produced using large-scale manufacturing, and
they are to be cryogenically stored (in multiple doses) until needed.

designed cold-responsive nanoparticles that release

also a strong need for better thawing technology.

trehalose intracellularly upon cooling, and offers the

" Thawing technology is important because thawing

additional benefit of skipping a cell washing step

cells too slowly can damage cells, and once cells

that can lead to cell loss.11

are thawed, DMSO is cytotoxic, " he points out. " The

A group of researchers at the University of

thawing process varies from center to center, but

Warwick led by Matthew I. Gibson, PhD, also demon-

most protocols involve rapid thawing of the cells in a

strated that extracellular deposition of naturally

water bath with manual manipulation of the cells. "

occurring type III antifreeze proteins significantly

Together with colleagues from China, Xiaoming

improves the post-thaw recovery of cells seeded in a

He synthesized magnetic nanoparticles14 and made

monolayer.12 Recently, Anne Meddahi-Pellé, MD, PhD,

use of magnetic induction heating to more uniformly

and her team from the University of Paris synthesized

thaw stem cells. The nanomaterials provided a

dextran-based hydrogels that demonstrated

spatially homogenous warming rate during thawing,

comparable cryoprotective ability as DMSO, without

enabling faster and more uniform melting of ice

the side effects, albeit in human cell lines.13

crystals.

According to David Stroncek from NIH, there is

Remarkably, stem cells that were thawed using

GENengnews.com

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Catalent eBook

Table of Contents for the Digital Edition of Catalent eBook

Contents
Catalent eBook - 1
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Catalent eBook - Contents
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