Catalent eBook - 27

Novel Approaches to Improve Cell Therapy Manufacturing

Steve G. Schmeissner / Getty Images

When chimeric antigen receptor (CAR) T cells are thawed after cryopreservation, quality assessments go well beyond
questions of viability. A comprehensive panel to investigate the impact of cryostoring cells on CAR T cells should include
general tests (such as proliferation, metabolism, and gene expression changes) and application-specific tests (such as
differentiation potential and cytotoxicity).

For CAR T cells, a combination of in vitro and

Conclusions

in vivo assays is necessary to examine the effects

The field of cell therapy is evolving rapidly with new

of cryopreservation.18 Some of the in vitro assays

therapeutic targets and with better techniques to

include CAR T-cell transwell migration, cytokine

transfect cells19 and enhance safety.20 As the popu-

production, and cytotoxicity measurements.

lation ages, the demand for cell-based regenerative

Common in vivo tests include CAR T-cell

medicine, biobanking, and tissue transplants is also

infiltration into tumor tissues (number and spatial

likely to increase. These trends will intensify the

distribution) and survival profiles of animal

need for better ways to characterize the impact of

models and humans. A comprehensive panel

cryopreservation technology to improve cryostoring

to investigate the impact of cryostoring cells

and thawing. Innovations in cryopreservation will

should include general tests (such as proliferation,

also benefit biomedical research by enhancing

metabolism, and gene expression changes) and

current protocols and tools to preserve organoids21

application-specific tests (such as differentiation

and xenografts, which are becoming more popular in

potential and cytotoxicity).

drug screening and basic science research. n

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Catalent eBook

Table of Contents for the Digital Edition of Catalent eBook

Contents
Catalent eBook - 1
Catalent eBook - 2
Catalent eBook - 3
Catalent eBook - Contents
Catalent eBook - 5
Catalent eBook - 6
Catalent eBook - 7
Catalent eBook - 8
Catalent eBook - 9
Catalent eBook - 10
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Catalent eBook - 12
Catalent eBook - 13
Catalent eBook - 14
Catalent eBook - 15
Catalent eBook - 16
Catalent eBook - 17
Catalent eBook - 18
Catalent eBook - 19
Catalent eBook - 20
Catalent eBook - 21
Catalent eBook - 22
Catalent eBook - 23
Catalent eBook - 24
Catalent eBook - 25
Catalent eBook - 26
Catalent eBook - 27
Catalent eBook - 28
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