EMD Millipore eBook - 10

Defenders Against Death
A Review of Literature Describing How Researchers
Used Intracellular Combinatorial Libraries to Select
Antibodies that Protected Cells From Death
ANALYZING CELL VIABILITY

Anton Simeonov, Ph.D.

T

he infective antibody library platform
developed by the Lerner team approximately
two years ago, in which lentiviral infection of
a mammalian cell host by an antibody library
effectively links antibody genotype and cell
phenotype, has been used in several successful
screens, delivering antibodies with unique
properties such as those having the ability
to transdifferentiate cells without the need
for transitional induced pluripotency. In this
10

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article published in Chemistry & Biology, vol. 21,
the team uses the platform to find antibody tools
to dissect a fundamental process of cell death
brought about by an acute viral infection.
An iterative selection was devised in which
antibody genes recovered from surviving cells
were used for each new round. A 108-member
naive antibody lentivirus library was used to
induce cytoplasmic expression of antibodies
in HeLa cells prior to infecting them with

"One of the most important phenotypes in biology is cell death."
Anton Simeonov, Ph.D., NIH
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