GEN-Thermo_Oct22_MakingThePromiseOf - 29

Part 1.
mRNA characterization
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Direct mRNA Sequence Confirmation
End-to-end LC-HRAM MS solution
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mRNA
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RNase T1
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Reproducible, controlled partial digestion
using immobilized RNAse magnetic beads.
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100
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RNA-Seq is the most
common technology
* Indirect. mRNA needs to be converted into
DNA.
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* Library/primer design is needed for each
mRNA.
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* Analysis takes >2 days.
* Multi-step, multi-instrument process.
An innovative LC-HRAM
MS workflow
* Direct measurement of mRNA.
* Universal, no need for library/primer.
* Fast (~2 hr), reproducible.
* Accurate, comprehensive sequence
coverage (>85%).
* End-to-end solution with automation.
Confident, comprehensive sequence identification.
GENengnews.com | 29
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5
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RT (min)
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High-resolution separation and high-quality MS, MS/MS data.
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4.77
5.17
10.7611.78
9.66
6.51 8.08
9.00
14.81
15.80
29.60
13.99
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24.96
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17.97
17.22
20.73
18.73
19.70
26.70
27.64
31.81
33.24
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34.8936.09
39.49
40.66
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23.69
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28.30
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22.78
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Relative intensity
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GEN-Thermo_Oct22_MakingThePromiseOf

Table of Contents for the Digital Edition of GEN-Thermo_Oct22_MakingThePromiseOf