Analytical Solutions for mRNA Vaccines and Therapeutics U Direct mRNA Sequence Confirmation Step 1: reproducible, controlled digestion U U U U U mRNA U U U U U U U U U U RNase T1 U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U Reproducible, controlled parUtial digestion using immobilized RNAse magnetic beads. U U U U U U U U * Controlled partial digestion through immobilized RNAse avoids over-digestion, generates large fragments with unique sequences. U U U U U U U U * Fast and complete removal of RNAse at the end of digestion eliminates system contamination. U U U U U U U U U U U U U U U U * Digestion can be automated using the Thermo Scientific™ KingFisher™ Duo Prime purification system magnetic bead robot for high reproducibility and throughput. U KingFisher Duo Prime Well plate 100 20 40 60 80 100 20 40 60 80 100 20 40 60 80 5 10 15 20 25 30 Time (min) Chromatograms of three replicate eGFP mRNA digests show highly reproducible digestion. 30 | GENengnews.com 35 40 45 50 Replicate 3 Replicate 2 U U U U U U U U U U U U U U U U U U U U U U U U KingFisher Duo Prime purification system eGFP mRNA Replicate 1 Relative abundance Relative abundance Relative abundance U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U Uhttps://www.genengnews.com https://www.thermofisher.com/order/catalog/product/5400110?SID=srch-srp-5400110 https://www.genengnews.com