GEN-Thermo_Oct22_MakingThePromiseOf - 31

OVERCOMING mRNA PRODUCTION SCALE-UP CHALLENGES
Direct mRNA Sequence Confirmation
Step 2: reproducible, high-quality LC-HRAM MS analysis
U
U
U
U
U U
U
U U U
U U
U
U
U
U U
mRNA digest
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U
U
U U
U
U
U
Reproducible, controlled partial digestion using
immobilized RNAse magnetic beads.
U
100
U
Total ion chromatogram
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U
U
U U
80
U U
60
1842-1853
125-144
1074-1105
40
928-943
843-855
20
445-457
1636-1649
1902-1914
1162-1178
1544-1558
145-160
514-527
458-475
476-495
675-732
384-400
1934-1993
1429-1484
1435-1514 263-290
5
10
-8
777.6240
w4
1243.1938
100
MS
80
60
-7
40
20
600
700
800
900
1000
1100
m/z
High-resolution separation and high-quality MS, MS/MS data.
1200
1300
1400
1500
1600
600
800
1000
1200
m/z
1400
1600
1800
-9
691.1094
-6
1037.1680
-5
1245.0037
20
w2
650.1019
888.8570
40
-4
1556.2557
w6
1901.2972
a6-B
1686.2902
80
60
100
a3-B
739.1392
954.1474
a4-B
1068.1919
w3
a5-B
1357.2377
M-G²
-
1465.2437
MS/MS
w5
1572.2457
14
20
RT (min)
25
30
35
40
91-164
604-652
2407-2435
692-732
1376-1428
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1609-1628
188-208
U
2221-2254
U U U
458-495
1465-1483
1214-1237
1485-1516
1536-1575
745-784
689-726 1376-1428
579-631
U
223-268
2060-2109
* Confident sequence identification
is ensured by high-quality MS, MS/MS
spectra acquired on Thermo Scientific™
Orbitrap Exploris™ mass spectrometers.
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41-76
U U223-262
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U
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* High-resolution separation of RNA
fragments is achieved at a high pH using
Thermo Scientific™ DNAPac™ RP HPLC
columns with unique polymeric structure.
* Robust and reproducible separation
is ensured by using the biocompatible
Thermo Scientific™ Vanquish™ UHPLC
system.
Thermo Scientific™
DNAPac™ RP HPLC Columns
Vanquish Flex 300
32.25 H x 21.65 W
Thermo Scientific™ Vanquish™
Flex UHPLC System
Thermo Scientific™ Orbitrap
Exploris™ 240 Mass Spectrometer
Orbitrap Exploris 240
31
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Relative abundance
Relative intensity
Relative abundance

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GEN-Thermo_Oct22_MakingThePromiseOf

Table of Contents for the Digital Edition of GEN-Thermo_Oct22_MakingThePromiseOf