GEN-Thermo_Oct22_MakingThePromiseOf - 32

Analytical Solutions for mRNA Vaccines and Therapeutics
Direct mRNA Sequence Confirmation
Step 3: automated, streamlined data processing
* Confident oligo sequence identification using MS/MS spectra of multiple charge states and
innovative kinetic prediction algorithm.
* Intuitive user interface supports customized building blocks and modifications.
* Accurate identification and separation of sequence isomers.
* Comprehensive sequence coverage at >85%.
100
10
20
30
40
50
60
70
80
90
5
10
14
20
RT (min)
25
30
35
40
41-76
223-262
-6
100
2221-2254
1609-1628
188-208
1842-1853
125-144
1074-1105
1544-1558
928-943
843-855
445-457
1636-1649
1902-1914
1162-1178
145-160
514-527
458-475
476-495
675-732
384-400
1934-1993
1429-1484
1435-1514 263-290
Z-7
Bf
Thermo Scientific™
BioPharma Finder™
Software
Z-6
Z-5
Z-4
Z-3
91-164
500
500
600
600
700
800
800
900
900
1000
1000
m/zm/z
1100
1100 12001200 1300 13001400
1400
1500
1500
604-652
2407-2435
692-732
1376-1428
1465-1483
458-495
1214-1237
1485-1516
1536-1575
745-784
689-726 1376-1428
579-631
223-268
2060-2109
100
20
40
60
80
-7
20
40
60
80
592.0774
592.0774
-7
-3
-3
-5-5
829.3109
517.9420
517.9420
629.3591
629.3591
829.3109
-4
906.7110
906.7110
-4
1036.8909
1036.8909
1244.1411
1244.1411
1469.5143
1469.5143
1382.8563
1382.8563
690.9248
690.9248
-6
Improved sequence identification using HRAM MS/MS spectra of multiple
charge states.
5.45
Isomers with identical
theoretical monoisotopic
mass (3532.4954 Da)
5'-AACAGCCCAUGcP-3'
5'-AACAGCCCAUGcP-3'
100
100
80
5'-UAAGCCACACGcP-3'
80
5'-UAAGCCACACGcP-3'
60
60
40
40
20
20
5
Confident sequence identification and comprehensive
sequence coverage.
5
10
Sequence isomers are baseline separated and confidently identified
using high-quality HRAM MS/MS spectra.
10
4.28
4.28
5.45
32 | GENengnews.com
Relative intensity
Relative abundance
Relative abundance
Relative abundance
Relative abundance

https://www.genengnews.com https://www.thermofisher.com/order/catalog/product/OPTON-30986?SID=srch-srp-OPTON-30986 https://www.genengnews.com

GEN-Thermo_Oct22_MakingThePromiseOf

Table of Contents for the Digital Edition of GEN-Thermo_Oct22_MakingThePromiseOf