Part 2. Lipid Nanoparticle Characterization LNP composition analysis by LC-CAD Fast high-resolution separation of LNP components was achieved on the Thermo Scientific™ Accucore™ C30 column in 10 minutes. Sensitive detection and accurate quantification of all the components were obtained using Thermo Scientific™ Vanquish™ charged aerosol detector, the industry standard platform for lipid identity, lipid content and lipid impurity. Charged aerosol detector provides universal detection with wide dynamic range up to 105 , and allows quantification of low-level impurities and high-level API in a single run. + Buspirone L-Ascorbic acid New coccine Buspirone L-Ascorbic acid New coccine Substances D-Glucose Lactose Diclofenac sodium salt Substances D-Glucose Lactose Diclofenac sodium salt L-Citruline Fluorescein Cortisone L-Citruline Fluorescein Cortisone Base-line separation of lipid components and minor impurities 10.0 20.0 30.0 40.0 50.0 60.0 DOPE 10.0 20.0 30.0 40.0 50.0 60.0 0.0 0.0 1.0 2.0 0.0 0.0 1.0 2.0 Universal response across broad range of molecules CAD response to 0.5 µg by flow injection 5 5 CAD response to 0.5 µg by flow injection 3.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 16.0 Time (min) 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 16.0 Time (min) PEG 5000 Cholesterol PEG 5000 Lipid H Cholesterol Lipid H DOPE View complete application note + PEG-lipid Cholesterol Lipid H DOPE Thermo Scientific™ Accucore™ C30 HPLC Columns 36 Thermo Scientific™ Vanquish™ System with Charged Aerosol Detector Vanquish Flex 300 32.25 H x 21.65 W Vanquish Flex 300 32.25 H x 21.65 W + Peak area [pA *min] Peak area [pA *min] Signal (pA) Signal (pA) + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +https://assets.thermofisher.com/TFS-Assets/CMD/Application-Notes/an-000465-uhplc-cad-lipid-nanoparticle-composition-an000465-na-en.pdf https://www.thermofisher.com/order/catalog/product/VF-D20-A?SID=srch-hj-VF-D20-A https://www.thermofisher.com/order/catalog/product/27826-152130?SID=srch-hj-27826-152130 https://www.thermofisher.com/order/catalog/product/VF-D20-A?SID=srch-hj-VF-D20-A https://www.thermofisher.com/order/catalog/product/27826-152130?SID=srch-hj-27826-152130