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Innovations in Cell-Based Screening * Finding a Signal Amid the Noise

References
1. Zhou Y., et al. High-throughput screening of a CRISPR/Cas9 library for
functional genomics in human cells. Nature 509, 487-491 (2014)

10. Qi LS., Repurposing CRISPR as an RNA-guided platform for sequence-specific
control of gene expression. Cell 152, 1173-1183 (2013)

2. Smith C., et al. Whole-genome sequencing analysis reveals high specificity of
CRISPR/Cas9 and TALEN-based genome editing in human iPSCs. Cell Stem Cell.
15, 12-13 (2014)

11. Sanjana N.E., Shalem O., & Zhang F. Improved vectors and genome-wide
libraries for CRISPR screening. Nat. Methods 11, 783-784 (2014)

3. Yang L., et al. CRISPR/Cas9-Directed Genome Editing of Cultured Cells. Curr.
Protoc. Mol. Biol. 107, 1.1-31.1.17 (2014)
4. Platt R.J., et al. CRISPR-Cas9 Knockin Mice for Genome Editing and
Cancer Modeling. Cell 159, 440-55 (2014)
5. Xue W., et al. CRISPR-mediated direct mutation of cancer genes
in the mouse liver. Nature 514, 380-4 (2014)
6. Yang H., Wang H., & Jaenisch R. Generating genetically modified mice using
CRISPR/Cas-mediated genome engineering. Nat. Protoc. 9, 1956-1968 (2014)

12. Hsu P.D., Lander E.S., & Zhang F. Development and applications of CRISPR-Cas9
for genome engineering. Cell 157, 1262-1278 (2014)
13. Kim H., & Kim J.S. A guide to genome engineering with programmable
nucleases. Nat Rev Genet. 15, 321-334 (2014)
14. Sander J.D., & Joung J.K. CRISPR-Cas systems for editing, regulating and
targeting genomes. Nat Biotechnol. 32, 347-355 (2014)
15. Doench J.G., et al. Rational design of highly active sgRNAs for CRISPR-Cas9mediated gene inactivation. Nat Biotechnol. Sep 3 2014. doi: 10.1038/nbt.3026.

7. Koike-Yusa H, et al. Genome-wide recessive genetic screening in mammalian
cells with a lentiviral CRISPR-guide RNA library. Nat Biotechnol., 32, 267-273 (2014)
8. Shalem O., et al. Genome-scale CRISPR-Cas9 knockout screening in human cells.
Science 343, 84-87 (2014)
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| GENengnews.com

Nicola McCarthy (n.mccarthy@horizondiscovery.com)
is the oncology program manager at Horizon Discovery.



http://www.GENengnews.com

Horizon eBook

Table of Contents for the Digital Edition of Horizon eBook

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