Immuno-Oncology Advances in Applied Cellular Analysis - 18

Advances in Applied Cellular Analysis * Continuous Live-Cell Analysis of the Immune-Tumor Axis

readouts, flow cytometry, and in some cases
high-content imaging.
Most recently, continuous live-cell analysis
(CLCA) has emerged as a powerful addition to
the line-up. CLCA is a non-invasive cell monitoring and measurement method based on
time-lapse, phase-contrast microscopy and
fluorescence imaging (e.g., IncuCyte®, Figures
1-3). In contrast to other methods, IncuCyte
CLCA allows visualization and direct quantification of the full timecourse of the biology of
interest rather than relying on arbitrary end
point measures. Moreover, the approach does
not require cells to be removed from the incubator and lifting, washing, or labeling with
perturbing (and expensive) antibodies is not
necessary. Together, this eliminates a wide
range of potential assay artifacts and affords
significant additional biological insight.
Interrogation of the timelapse movies allows
researchers to observe changes in morphology,
movement, and spatial orientation of cancer
and immune cells over days and weeks. Analysis is fully automated and assays are medium
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IncuCyte® S3 Live-Cell
Analysis System
A fully-automated phase-contrast
and two-color fluorescence imager
that resides within a standard cell
incubator for optimal cell viability.
Designed to scan plates and flasks
repeatedly over time.

IncuCyte® Software
Fast, flexible, and powerful control
hub for continuous live-cell analysis
comprising image acquisition,
processing, and data visualization.

Figure 1. IncuCyte® system for continuous live-cell analysis.

IncuCyte® Reagents and
Consumables
A suite of non-perturbing cell
labeling and reporter reagents.
Includes nuclear-targeted GFP
and RFPs for cell counting, nowash caspase 3/7 substrate for
apoptosis and cell kits for
angiogenesis.

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Immuno-Oncology Advances in Applied Cellular Analysis

Table of Contents for the Digital Edition of Immuno-Oncology Advances in Applied Cellular Analysis