Immuno-Oncology Advances in Applied Cellular Analysis - 24

Advances in Applied Cellular Analysis * Continuous Live-Cell Analysis of the Immune-Tumor Axis

CCRF-CEMs, albeit over longer time periods
(i.e., 24h vs 4h). This assay is suitable for profiling
novel CD47 modulators.

CXCR4-Receptor Mediated
Chemotaxis of Human T-Cells
Tumor-associated chemokines, such as the
CXCR4 ligand CXCL12, play a central role in
cancer biology, promoting leukocyte infiltration,
tumor growth, and immune evasion. CXCR4mediated chemotaxis (gradient-dependent directional movement) is a key mechanism by which
T-lymphocytes and other immune cells are drawn
toward the tumor microenvironment. However,
tumor cells are able to hijack the chemokine
receptor/chemokine system by switching infiltrating
leukocytes from immuno-attack to immunotolerance.5 Accordingly, inhibitors of CXCR4 are of great
interest as novel immuno-oncology therapeutics.
CLCA chemotaxis assays were conducted using
IncuCyte® ClearView 96-well plates, a novel transwell consumable that incorporates precision, laserdrilled pores in each well for cells to move through
(Figure 7). Human T-lymphocytes (5K cells per well)
were added to the upper wells of fibronectin-coated
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| GENengnews.com

Figure 7 (A & B).
IncuCyte continuous live-cell assay for CXCR4mediated chemotaxis of human T-lymphocytes.
Label-free assays were performed on 96-well IncuCyte
ClearView plates to enable visualization of the
transmigration of cells (e.g., red arrow) through
laser-drilled pores (yellow circles) toward the
SDF-1α chemo-attractant.

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Immuno-Oncology Advances in Applied Cellular Analysis

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