Immuno-Oncology Advances in Applied Cellular Analysis - 26

Advances in Applied Cellular Analysis * Continuous Live-Cell Analysis of the Immune-Tumor Axis

quantitative, and informative cellular assays
based on direct phenotypic outcomes (killing,
engulfment, directional movement). The key
attributes of CLCA are fourfold: (1) the breadth
and versatility of the types of assays and measures that can be made, (2) the high relevance
borne of measurements made non-invasively
and from living cells, (3) the additional biological insight gained from images and movies over
time, and (4) the enhanced productivity provided
by fully automated image capture and analysis.
Moreover, unlike most microscope systems,
IncuCyte is able to readily image and quantify
non-adherent cells over long time periods thus
allowing studies on hematologic cells. Together,
these features enable researchers to probe and
quantify the interplay between immune and
cancer cells in ways not covered by other methodologies. Importantly, CLCA affords trustworthy
and reliable analysis of the activities of new
potential immune-therapies. CLCA and IncuCyte®
is fast becoming an essential and obligate method
in the immuno-oncology research field. n

| GENengnews.com

References
1. R. Houot et al., Cancer Immunol. Res. 3, 1115-1122 (2015).
2. E. McCormack et al., Cancer Immunol. Immunother. 62, 773-785 (2012).
3. K. Weiskopf et al., J. Clin. Invest. 126, 2610-2620 (2016).
4. M. S. Chaor et al., Cell 142, 699-713 (2010).
5. M. Vela et al., Front. Immunol. 6, article 12 (2015).

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Immuno-Oncology Advances in Applied Cellular Analysis

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