or immuno-oncology research and the hunt for new immunotherapies, scientists are required to reliably quantify the functions and interactions of cancer cells and immune cells. Cell proliferation assays of tumor growth are a mainstay. Activation and proliferation assays for immune cells are also used to study how the body mobilizes its forces against invading cancers. Readouts of cell migration, the underlying process for the spread and invasion of tumors as well as the directional movement of immune cells toward tumors are also key. Perhaps most importantly, co-culture assays are deployed to quantify the killing and clearance of tumor cells by the immune system. Essen BioScience is a world leader in quantitative cell-based assay solutions. Our IncuCyte® Live-Cell Analysis System is a powerful and flexible technology that enables real-time, automated and direct measurements of the dynamic interactions between immune and cancer cells. The system fits seamlessly in to existing research workflows. Here, we share a series of short articles describing new and exciting developments in the field of immuno-oncology. These include illustrations of how real-time live-cell analysis provides an invaluable and enabling toolkit of assays and reagents for immuno-oncology researchers. We hope you find this e-book a valuable and educational resource as well as an enjoyable read!http://www.essenbioscience.com