LGC eBook - 2017 - 22

Accelerating Science Using Integrated PCR Tools * Point-of-Care Molecular Diagnostics Evolve

et al., writing in the August 2013 issue of PLOS
One, demonstrated the detection of the HIV-1
integrase gene with the µBAR using LAMP. Since
LAMP has previously been demonstrated to work
with a range of clinical samples, the scientists say
their eventual goal is to develop a microfluidic
device which includes on-chip sample preparation from raw samples. The authors note that
their goal in developing the instrument was to
introduce a platform for isothermal amplification
and real-time detection on a multiplexed
microfluidic cartridge.
The device improves on the state-of-the-art in
genetic amplification instrumentation in its cost,
operational simplicity, and portability. They point
out that their instrument could be conceivably
priced ~50× cheaper than conventional real-time
thermocyclers. It does not require external
power, and at least three assays can be run before
the internal battery requires recharging.

| GENengnews.com

In its present form, they concede, the µBAR
device does not provide sample-prep capabilities and requires that amplification reagents be
mixed off-chip before sample loading. It also
requires that DNA be supplied at fairly high
concentrations (1,000/uL, for example) because
of the small volumes used in the microfluidic
chamber. To help further development of POC
genetic diagnostic instruments, the authors have
provided designs, source code, and instructions
for µBAR instruments.
Investigators and clinicians anticipate that given
market forces and current demand in specific
markets, most notably infectious diseases,
portable, hand-held, inexpensive POC devices,
equivalent to currently offered full-size molecular
analytical systems, will become available. These
devices will aid in the detection of mutations or
in the identification of infectious agents, as well
as aid in the management of patient care. n

http://www.GENengnews.com

LGC eBook - 2017

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