LGC eBook - 2017 - 8

High-Multiplex
Real-Time PCR Design
Utilizing the Tagging Oligonucleotide
Cleavage and Extension (TOCE) Technique
Sara Agee, Ph.D.
To meet challenges such as these, Seegene has
developed a new method for high-multiplex
real-time PCR technology. This method, which
is called Tagging Oligonucleotide Cleavage
and Extension (TOCE™), enables detection of
multiple targets in a single fluorescence channel through melting temperature analysis of a
series of artificial templates. TOCE can enable
multiplex assays to be run on existing real-time
PCR instrumentation.

Multiplex assay development is notorious for
presenting certain difficulties, including multiplex probe design, oligo interference, assay
design flexibility, and optimization.

TOCE is a novel approach to real-time PCR and
has several unique oligonucleotide components (Figure 1). The key components for TOCE
technology are dual-priming oligonucleotide

8

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T

he real-time polymerase chain reaction (PCR)
has become a key tool for molecular assay
development, expanding into applications as
diverse as in vitro diagnostics (IVDs), food safety
testing, and pharmacogenomics. Now, real-time
PCR instrumentation systems with multiple fluorescence detection channels have expanded the
capacity of PCR to detect multiple analytes in a
homogeneous assay, increasing the information
gathered per reaction with less sample input.

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LGC eBook - 2017

Table of Contents for the Digital Edition of LGC eBook - 2017