PerkinElmer eBook - Next-Gen Sequencing - 22

Next-Gen Sequencing * What's Next for Next-Gen Sequencing?

TLA enables the targeted sequencing as well
as the haplotyping of any region of interest
in any genome, irrespective of the sequence
changes, and it provides comprehensive
sequencing information about the presence
of single nucleotide variants in addition to
structural variants. The technology is highly
suited for transgene analysis to determine
integration sites, and to verify whether
transgenes have really integrated in the
appropriate manner.
TLA uses one primer pair, specific for the region of interest, for the targeted amplification.
For the transgene application, primers specific
for sequences that occur only in the transgene
are used. Then the protocol provides
complete sequencing information including
any genetic alterations that may have occurred
in the transgene and integration site.
According to Max van Min, CEO, Cergentis,
current applications based on the whole-cell
protocol involve the complete sequencing of
regions that may be diagnostically relevant or
linked to phenotypes of interest.
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| GENengnews.com

Examples include using TLA technology with
cancer cells to sequence genes of interest
and detect variation potentially linked to
prognosis and drug response, and supporting
pharmaceutical companies in the characterization of transgene model organisms and
cell lines for disease or drug-response
modeling, or cell lines for the production
of proteins and primary antibodies.
Since TLA provides more genetic information
than other technologies, the classical NGS challenge applies-how to interpret this additional
genetic information. In some cases, the genetic
variants of clinical interest are already known,
but occasions exist in which it is difficult to
classify a variant and determine whether it is
linked to the trait of interest or is just benign.
The first version of the TLA protocol uses
whole cells as the input material. A newly
developed protocol is compatible with
isolated DNA and opens up new opportunities in genetic diagnostics, enabling the
targeted sequencing of any region of interest
in microbial, plant, or animal genomes.

Cergentis' Targeted Locus Amplification (TLA) technology
presents unique advantages in the complete sequencing
of genes of interest and the detection of all gene fusions. It
uses two PCR primers complimentary to a short locus specific
sequence in the gene of interest and enables the amplification
and next-generation sequencing of the entire genomic locus
regardless of what point mutations and structural variations
occur in it.

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PerkinElmer eBook - Next-Gen Sequencing

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